Gene

Primer Sequence[5'-3']

Amplicon size(bp)

Intervening sequence size(bp)

Amplification efficiency(%)

ACTB

F: GGCACCCAGCACAATGAAG
R: CCGATCCACACGGAGTACTTG

66

178

98

GAPDH

F: AGATCCCTCCAAAATCAAGTGG
R: GGCAGAGATGATGACCCTTTT

130

220

98

GUSB

F: GAAAATATGTGGTTGGAGAGCTCATT
R: CCGAGTGAAGATCCCCTTTTTA

101

3360

93

HMBS

F: CACGATCCCGAGACTCTGCT
R: TACTGGCACACTGCAGCCTC

81

315

104

HPRT1

F: TGAGGATTTGGAAAGGGTGT
R: GAGCACACAGAGGGCTACAA

118

1833

99

TBP

F: GAGCTGTGATGTGAAGTTTCC
R: TCTGGGTTTGATCATTCTGTAG

117

1747

110

18S rRNA

F: GGAGTATGGTTGCAAAGCTGA
R: ATCTGTCAATCCTGTCCGTGT

129

_

89