Gene

Gene symbol

Primer sequence (5⁰ –3⁰ )

Amplicon length (bp)

T_m (LC)

Actin mRNA

ACT

For  TGTTATGGTAGGGATGGGTC

228

83.07

Rev  TTCTCTCTATTTGCCTTGGG

Ubiquitin-conjugating enzyme

UBI-1

For  AAGGAAATGTGTGTCTCAAC

278

85.16

Rev TCCAAATGCCAAACTTCTAG

Ubiquitin-conjugating protein

UBI-3

For  TGTCCATCTGCTCTCTGTTG

204

85

Rev CACCCCAAGCACAATAAGAC

Glyceraldehyde-3-phosphate dehydrogenase-GapCp

GAPDH

For ATGATGATGTGAAAGCAGCG

276

85

Rev  TTTCAACTGGTGGCTGCTAC

Eukaryotic initiation factor 5A2

EIF5A2

For  CCTGTTATCGTGCTACTTTG

112

80.24

Rev  GTTTCATTGCCNTGCCAGAT

Beta tubulin

b-TUB

For  GAGGGTGAGTGAGCAGTTC

167

83.81

Rev  CTTCATCGTCATCTGCTGTC

Alpha tubulin

a-TUB

For  ATCAACTATCAGCCACCAAC

175

85.01

Rev TACCCTCACCAACATACCAG