发布者：Primerbank 时间：2018-01-25 浏览量：2156
| Name | Primer Sequence | Tm |
| NS1 | GTAGTCATATGCTTGTCTC | 49 |
| CNS1 | GAGACAAGCATATGACTACTG | 55 |
| NS2 | GGCTGCTGGCACCAGACTTGC | 65 |
| NS3 | GCAAGTCTGGTGCCAGCAGCC | 65 |
| NS4 | CTTCCGTCAATTCCTTTAAG | {62} |
| NS5 | AACTTAAAGGAATTGACGGAAG | 55 |
| NS6 | GCATCACAGACCTGTTATTGCCTC | {72} |
| NS7 | GAGGCAATAACAGGTCTGTGATGC | {72} |
| NS8 | TCCGCAGGTTCACCTACGGA | 59 |
| TW9 | TAAGCCATGCATGTCT |
|
| TW10 | GCGGTAATTCCAGCTCC |
|
| TW11 | GGAGTGGAGCCTGCGGCT |
|
| TW12 | AAGTCGTAACAAGGTTT | 53 |
| CTW12 | AAACCTTGTTACGACTT | 53 |
| NS17 | CATGTCTAAGTTTAAGCAA | 55 |
| NS18 | CTCATTCCAATTACAAGACC | 60 |
| NS19 | CCGGAGAAGGAGCCTGAGAAAC | 74 |
| NS20 | CGTCCCTATTAATCATTACG | 61 |
| NS21-ag | GAATAATAGAATAGGACG | 50 |
| NS21-ls | AATATACGCTATTGGAGCTGG |
|
| NS22 | AATTAAGCAGACAAATCACT | 57 |
| NS23 | GACTCAACACGGGAAACTC | 64 |
| NS24 | AAACCTTGTTACGACTTTTA | 58 |
| NS25 | GTGGTAATTCTAGAGCTAATACT |
|
| CNS25 | ATGTATTAGCTCTAGAATTACCAC |
|
| NS26 | CTGCCCTATCAACTTTCGA |
|
| CNS26 | TCGAAAGTTGATAGGGCAG |
|
| VANS1 | GTCTAGTATAATCGTTATACAGG | 57 |
| MB1 | GGAGTATGGTCGCAAGGCTG |
|
| CMB1 | CAGCCTTGCGACCATACTCC |
|
| MB2 | GTGAGTTTCCCCGTGTTGAG | 57 |
| Basid 1 | TTGCTACATGGATAACTGTG | 49 |
| Basid 2 | CTGTTAAGACTACAACGG |
|
| Basid 3 | AGAGTGTTCAAAGCAGGC |
|
| Basid 4 | CTCACTAAGCCATTCAATCGG |
|
| NS1.5R | TCTAGAGCTAATACATGC(T/C)G | 52 |
| NS2.8R | GGCCCTCAAATCTAAGGATT | 53 |
| CNS2.8R | AATTTGCGCGCCTGCTGCAA | 57 |
| NS3.2R | CGTATATTAAAATTGTTGAC | 45 |
| CNS3.3R | GACTACGAGCTTTTTAACGT | 51 |
| CNS3.5R | TTTCGCAGTAGTTTGTCTTA | 49 |
| NS3.6R | CAAACTACTGCGAAAGCATC | 53 |
| CNS3.6R | AATGAAGTCATCCTTGGCAG | 53 |
| ITS1 | TCCGTAGGTGAACCTGCGG | 57 |
| ITS1-F | CTTGGTCATTTAGAGGAAGTAA | 55 |
| ITS1-P | TTATCATTTAGAGGAAGGAG | 49 |
| ITS1-P2 | CTTTATCATTTAGAGGAAGGAG | 55 |
| ITS1-R | (t/a)TGGT(c/t)(a/g/t)(t/c)AGAGGAAGTAA |
|
| ITS2 | GCTGCGTTCTTCATCGATGC | 57 |
| ITS2-Melampsora | CACTGTGTTCTTCATCGACG | 55 |
| ITS2-R | TGTGTTCTTCATCGATG | 43 |
| ITS3 | GCATCGATGAAGAACGCAGC | 57 |
| ITS3-R | ATCGATGAAGAACACAG | 43 |
| ITS4 | TCCTCCGCTTATTGATATGC | 53 |
| ITS4-R | CAGACTT(G/A)TA(C/T)ATGGTCCAG | 53 |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG | {69} |
| ITS6R | GACACTCAAACAGGTGTACC | 55 |
| ITS6R2 | TCATGACACTCAAACAGGTG |
|
| ITS4-B | CAGGAGACTTGTACACGGTCCAG | 67 |
| CTB6 | GCATATCAATAAGCGGAGG | 51 {64} |
| NL1f | TGGGTGGTAAATTCCATCTA | 51 |
| NL2f | GTGAAATTGTTAAAAGGGAAAC | 53 |
| CNL2f | GTTTCCCTTTTAACAATTTCAC | 53 |
| KJ1 | GGCGGTAAATTCCGTCC | 49 |
| KJ2 | GCTTGAAATTGTCGGGAGGG | 57 |
| TW13 | GGTCCGTGTTTCAAGACG | 53 {66} |
| CTW13 | CGTCTTGAAACACGGACC | 51 {66} |
| TW14 | GCTATCCTGAGGGAAACTTC | 55 {64} |
| CTW14 | GAAGTTTCCCTCAGGATAGC | {64} |
| TW15 | CTTGGAGACCTGCTGCGG | 55 {72} |
| CTW15 | CCGCAGCAGGTCTCCAAG | {72} |
| CNL12 | CTGAACGCCTCTAAGTCAG | 53 |
| Kim-Q | ACGCCTCTAAGTCAGAAT | 47 |
| Kim-Y | TCGCAGAGCGAACGAGAT | 51 |
| CTB11 | GCAGCAGGTCTCCAAGGTG | {71} |
| TB11 | CACCTTGGAGACCTGCTGC | {71} |
| NL13 | CAAGCGAACTTTCATCATGCACG | 63 |
| NL14 | CTTTAGAACAAGGGTTGAAC | 51 |
| CNL14 | GTTCAACCCTTGTTCTAAAG | 51 |
| 5S-Anderson | CAGAGTCCTATGGCCGTGGAT | 61 |
| 5S-Bruns | GCATCCCGTCCGATCTGCGCAG | 69 |
| Kim-Y | TCGCAGAGCGAACGAGAT | 51 |
| 5S-rhizoc | GTACTAACTAGGCGGCACTC | 57 |
来源https://nature.berkeley.edu/brunslab/tour/primers.html