发布者：Primerbank 时间：2018-01-26 浏览量：1190
| Locus | Primer sequence | Amplicon | Size (bp) of: | % of | |
| ORF | |||||
| Forward | Reverse | Sequenced | sequenced | ||
| gene fragment |
|
||||
| abcZ | 5 -CATCATTATTGTTTCCGTCCTGCC | 5 -GATGATTTCTTCAGGTCGTTTGA | 2053 | 884 | 46.33 |
| adk | 5 -CAAGCCGTGTAGAATCGTAAACCA | 5 -CTGCCCGTGGGTACGACCTTTTC | 738 | 610 | 94.57 |
| aroE | 5 -CGCCCCGCCTTTATGGAATATGA | 5 -AGCCATTTGACGATGCGGAACAT | 861 | 796 | 98.64 |
| fumC | 5 -GAGAAACGTATGAACACCCGCACC | 5 -CTTCGCATTGGGTCGGGTTGACTT | 999 | 856 | 61.76 |
| gdh | 5 -CTGCTTGCGCTGCGTTTTGCCAAT | 5 -TTGTGATTTCAGACGGCATATCCC | 1029 | 861 | 100 |
| glnA | 5 -GTGTTCAGACGGCATTGCTACTGA | 5 -CCGCATATTTTGCCATTTCCCTTT | 1491 | 1356 | 95.76 |
| gnd | 5 -TTAAATCTATAACGGAGCTTCCTG | 5 -TCTATTTCCTGCAAAACAAATGCC | 1533 | 1446 | 100 |
| gpdh | 5 -CCAAACAGGAAGCCGCACAGAATA | 5 -CTGAAACTTGAAAACATCGGGTTT | 1318 | 1023 | 99.42 |
| gpdhC | 5 -CTTTATTTCACAACCGGGAGACAA | 5 -GCAATGTGTTCAGACGGCATTTAC | 1078 | 992 | 99 |
| pdhC | 5 -GCAAACAGGCCGTCTGAAACATCA | 5 -GATGAACGAGAACTACACCCATC | 617 | 498 | 88.77 |
| pgi1 | 5 -GAAAACCTCAATCGGGAGCATACA | 5 -TCGATAAAAATGCCGTCTGAAAC | 1728 | 954 | 58.03 |
| pgi2 | 5 -TACAGAAAGGCGGCGGTACTTTTA | 5 -GCCGTTTGTTAGACTACATTCTGC | 1726 | 1618 | 98.6 |
| pgm | 5 -TTTACAGAGGCCAGATTAAATGCG | 5 -GGCGAAGCCATTCAAGAACTTTTA | 1017 | 906 | 65.65 |
| pilA | 5 -CGCTACAATACGCCCTATTTCAAG | 5 -TTTGCAGCACGCGCTTCACTTTTT | 1045 | 932 | 73.79 |
| pip | 5 -AAAACAAAAGCAGCTTCCAAACA | 5 -GGATTGTCAGAAGCAATATGGGAG | 1012 | 826 | 88.82 |
| ppk | 5 -AGCGTATGGGGGAAGTGCTTGTGC | 5 -CGTGGTCGATATGATACGCGAAGC | 1029 | 906 | 44.09 |
| pyrD | 5 -TTCCAAATGACGGCTGATTG | 5 -CGTTTACGCCCAGTTGTTCA | 1112 | 1005 | 100 |
| serC | 5 -ACAATCTTACCACCTCCAAAACGC | 5 -AAGCCTGCTGCAAGCCTAAAATCG | 1246 | 1105 | 100 |
http://jcm.asm.org/content/41/1/197.full.pdf