Williams Lab #

Name

Sequence

Species

Desciption

2193

Wbm0556-F

AGCCATTGTACAGCTATGGAGGA

W. bancrofti

Forward qPCR primer Wolbachia glutathione-sythase

2194

Wbm0556-R

TGACCCTTGGAACTCGTTTCAT

W. bancrofti

Reverse qPCR primer Wolbachia glutathione-sythase

2195

Wbm0415-F

GCAAAGGAGCTGCTAGATGAGAA

W. bancrofti

Forward qPCR primer Wolbachia glutaredoxin

2196

Wbm0415-R

CATCTAACTTCCCTTCCCTTTCAAG

W. bancrofti

Reverse qPCR primer Wolbachia glutaredoxin

2197

Wbm0127-F

GTGCCACGCTAGCTTCAAGG

W. bancrofti

Forward qPCR primer Wolbachia Peroxiredoxin

2198

Wbm0127-R

CCACTAACTTTTACGTTCTCCCAGAT

W. bancrofti

Reverse qPCR primer Wolbachia Peroxiredoxin

2199

Wbm0569-F

GGCAAAAACATGCTCGTAATCA

W. bancrofti

Forward qPCR primer Wolbachia ferredoxin

2200

Wbm0569-R

ATTTGGCCACTTTTGCGAGT

W. bancrofti

Reverse qPCR primer Wolbachia ferredoxin

2201

Wbm0145-F

CCTTGCACAATTTAACTACAGGCA

W. bancrofti

Forward qPCR primer Wolbachia surF1-family protein with 2225 R

2216

Wlb.0707(F)-I

CCAGAAGCTAATGGAAATGATATTTTT

W. bancrofti

Forward qPCR primer Wolbachia cold shock protein with 2222

2222

Wlb.0707(R)-I

ATTCCCTTTTCCTTGTTTTCTCCT

W. bancrofti

Reverse qPCR primer Wolbachia cold shock protein with 2216

2225

Wlb.0145(R)-I

GAGACAGTGCATGATAGCCGC

W. bancrofti

Reverse qPCR primer Wolbachia surF1-family protein with 2201 F

2228

Wlb.0685(F)-I

GGTGCAGGACCTGTTGGAAT

W. bancrofti

Forward qPCR primer Wolbachia thioredoxin-reductase

2229

Wlb.0685(R)-I

AGTGCATTGTCCTCCCGCT

W. bancrofti

Reverse qPCR primer Wolbachia thioredoxin-reductase

2232

Wlb.0507(F)-II

GTGCATGCGAAGGCTCTCTT

W. bancrofti

Forward qPCR primer Wolbachia ferrdoxin

2233

Wlb.0507(R)-II

CCAAGCCTTGACGTCTCTGTT

W. bancrofti

Reverse qPCR primer Wolbachia ferrdoxin

2459

High Mobility Protei F

CAAGCGGAGCATCAACATCA

B. malayi

Forward qPCR primer amplifies B malayi High Motility Protien primers

2460

High Mobility Protei R

CGGTGCATTCGGATCTTTG

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protien primers

2461

High Mobility Protei F1

GACGAAGAAAGTCAAAGATCCGAAT

B. malayi

Forward qPCR primer amplifies B. malayi High Motility Protein

2462

High Mobility Protei R1

CGAGATATATTATCCAGCAACCAGAA

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protein

2463

High Mobility Protei F2

ACGAAGAAAGTCAAAGATCCGAAT

B. malayi

Forward qPCR primer amplifies B. malayi High Motility Protein

2464

High Mobility Protei R2

CGAGATATATTATCCAGCAACCAGAA

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protein

2465

High Mobility Protei F3

ACCATCATGGCTAAGACAGGGCCA

B. malayi

Forward qPCR primer amplifies B. malayi High Motility Protein

2466

High Mobility Protei R3

ACTTGGCTCGCTTCGGTGCA

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protein

2467

High Mobility Protei F4

CCATCATGGCTAAGACAGGGCCA

B. malayi

Forward qPCR primer amplifies B. malayi High Motility Protein

2468

High Mobility Protei R4

GCTCGCTTCGGTGCATTCGG

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protein

2469

High Mobility Protei F5

AGGTCAAGCGGAGCATCAACATCA

B. malayi

Forward qPCR primer amplifies B. malayi High Motility Protein

2470

High Mobility Protei R5

ACTTGGCTCGCTTCGGTGCAT

B. malayi

Reverse qPCR primer amplifies B. malayi High Motility Protein

2471

Major Sperm Protien2 F

CGTGTGACACATTCGATGCA

B. malayi

Forward qPCR primer amplifies B. malayi Major Sperm Protein2 primers

2472

Major Sperm Protien2 R

CATTCGGATGGTAATACGATCGT

B. malayi

Reverse qPCR primer amplifies B. malayi Major Sperm Protein2 primers

2473

Major Sperm Protien2 F1

CCACCGGGTGATATCCATACC

B. malayi

Forward qPCR primer amplifies B. malayi Major Sperm Protein2

2475

Major Sperm Protien2 R1

CGACCACCGGCATTAGTAATCTTAAT

B. malayi

Reverse qPCR primer amplifies B. Malayi Major Sperm Protein2 use with 2473

2476

Major Sperm Protien2 F3

GCAGTCGGTTCCACCGGGTG

B. malayi

Forward qPCR primer amplifies B. malayi Major Sperm Protein2

2477

Major Sperm Protien2 R2

CAATGCGACGACCACCGGCA

B. malayi

Reverse qPCR primer amplifies B. malayi Major Sperm Protein2 use with 2473

2478

Major Sperm Protien2 F4

CGCAGTCGGTTCCACCGGG

B. malayi

Forward qPCR primer amplifies B. malayi Major Sperm Protein2

2479

Major Sperm Protien2 R3

CCAATGCGACGACCACCGGC

B. malayi

Reverse qPCR primer amplifies B. malayi Major Sperm Protein2

2481

Major Sperm Protien2 R4

ACCAATGCGACGACCACCGG

B. malayi

Reverse qPCR primer amplifies B. malayi Major Sperm Protein2

2482

Actin 2B F

CTCCCGAGGAACATCCAGTACT

Forward qPCR primer contol primers

2483

Actin 2B R

CTCTGTTTGCCTTTGGGTTCA

Reverse qPCR primer contol primers

2484

NADH SubI F

GGGTGGCACTCAGTGTCGTA

Forward qPCR primer contol primers

2485

NADH SubI R

ACAACGCCTGAAAAATACCAGAGTA

Reverse qPCR primer contol primers

2486

Histone 3 F

GCTAACGAAAGCACCATCAAGA

Forward qPCR primer contol primers

2487

Histone 3 R

TCTTGCAAAGCACGCATACC

Reverse qPCR primer contol primers

2490

D.imm-F1

CATCAGGTGATGATGTGATGAT

D. immitis

Forward primer amplifies D. immitis ITS2

2491

D.imm-R1

TTGATTGGATTTTAACGTATCATTT

D. immitis

Reverse primer amplifies D. immitis ITS2

2492

DIDR-F1

AGTGCGAATTGCAGACGCATTGAG

Filaria

Forward primer amplifies filarial ITS2

2493

DIDR-R1

AGCGGGTAATCACGACTGAGTTGA

Filaria

Reverse primer amplifies filarial ITS2

2576

Bm1_juvp120_F

GCCTTTTTCACATTAACTCAGACA

B. malayi

Forward primer amplifies B malayi Juvp-120

2577

Bm1_juvp120_R

GCTGCTGAATAAGTGGTTGTTG

B. malayi

Reverse primer amplifies B malayi Juvp-120

2611

Mos ITS2 F

TGT GAA CTG CAG GAC ACA TGA A

Mosquito

Forward primer amplifies mosquito ITS2

2612

Mos ITS2 R

ATG CTT AAA TTT AGG GGG TAG TC

Mosquito

Reverse primer amplifies mosquito ITS2

2643

SSU R26

CATTCTTGGCAAATGCTTTCG

Filaria

Reverse primer amplifies filarial SSU with G18S4-SSU-F

2656

COI filaF

TDTCTWTRDTDATTCGTT

Filaria

Forward degenerate primers for filarial CoxI

2657

COI filR

ACDACATAATAAGTATCATG

Filaria

Reverse degenerate primers for filarial CoxI

2658

Fila12s F

CGGGAGTAAAGTTTTGTTTAAACCG

Filaria

Forward primer amplifies filarial 12s

2659

Fila12s R

CATTGACGGATGGTTTGTACCAC

Filaria

Reverse primer amplifies filarial 12s

2660

NC1- ITS2 F

ACGTCTGGTTCAGGGTTGTT

Filaria

Forward primer for filarial ITS2- designed for C.elegans

2661

NC2- ITS2 R

TTAGTTTCTTTTCCTCCGCT

Filaria

Reverse primer for filarial ITS2- designed for C.elegans

2662

ITS- A F

GGAAGGAGAAGTCGTAACAAGG

Forward primer for ITS

2663

ITS- B R

CTTTTCCTCCGCTTATTGATATG

Reverse primer for ITS

2664

ITS- C R

GCAATTCACACCAAGTATCGC

Reverse primer for ITS

2665

ITS- D F

CTCTCGGCAACGGATATCTCG

Forward primer for ITS

2666

ITS- E

CGGCAACGGATATCTCGGCTC

ITS primer

2667

ITS1

TCCGTAGGTGAACCTGCGG

ITS primer

2668

ITS2

GCTGCGTTCTTCATCGATGC

ITS primer

2669

16sOvC F

AGCCTTAGCGTGATGGCATA

O.volvulus

Forward primer for Ov SSU

2792

WSP F1

GTCCAATARSTGATGARGAAAC

Forward primer for WSP

2793

WSP R1

CYGCACCAAYAGYRCTRTAAA

Reverse primer for WSP

2802

16S UF

CCAGTGGCGAAGGCGTCTAT

Bacteria (universal)

Forward primer for Universal bacterial 16S primer

2803

16S UR

CCCCGTCAATTCCTTTGAGTTT

Bacteria (universal)

Reverse primer for Universal bacterial 16S primer

2810

16S Wolb F

GAAGATAATGACGGTACTCAC

W. bancrofti

Forward primer for 16S Wolbachia primer

2811

16S Wolb R

GTCACTGATCCCACTTTAAATAAC

W. bancrofti

Reverse primer for 16S Wolbachia primer

2812

FtsZUNI-F

GGYAARGGTGCRGCAGAAGA

W. bancrofti

Forward primer for FtsZ Wolbachia primer

2813

FtsZUNI-R

ATCRATRCCAGTTGCAAG

W. bancrofti

Reverse primer for FtsZ Wolbachia primer

2814

WSPintF

TAGYTACTACATTCGCTTGCA

W. bancrofti

Forward primer for WSP Wolbachia primer

2815

WSPintR

CCAAYAGTGCYATAAAGAAC

W. bancrofti

Reverse primer for WSP Wolbachia primer

2878

Lin42 cds forward

AAC GTG GCA CCA TCA GC

C. elegans

Forward primer for C.elegans Lin-42 complete coding sequence

2879

Lin42 cds reverse

CGC AAT CTC GAG ATA CTG TAG TAG A

C. elegans

Reverse primer for C.elegans Lin-42 complete coding sequence

2884

ITS2 Forward

TGTGAACTGCAGGACACATGAA

Mosquito

Forward primer for Anopheles ITS

2885

ITS2 Reverse

ATGCTTAAATTTAGGGGGTAGTC

Mosquito

Reverse primer for Anopheles ITS

3056

LCO1490

ggtcaacaaatcataaagatattgg

Mosquito

Mosquito Barcoding primers

3057

HC02198

taaacttcagggtgaccaaaaaatca

Mosquito

Mosquito Barcoding primers

3058

BmGAPDH F

ATGAGCAAACCAAAGGTTGGA

B. malayi

Forward primer for Bm GAPDH gene

3059

BmGAPDH R

TTATCTGCTGGCAATGTACG

B. malayi

Reverse primer for Bm GAPDH gene

2670A

16sOvB R

ACCCACATTGCATTCCTTTC

O.volvulus

Reverse primer for Ov SSU