Target genes

Primer sequence

Product size (bp)

Function

ftsZ

F: 5-GCGCACATGGTGTTCATTAC-3

192

cell division protein FtsZ

R: 5-GTCGACGTGATCCTCCAGTT-3

recA

F:5-CGTGAAGGTCGTCAAGAACA-3

166

recA protein DNA replication,recombination, and repair

R:5-GATCTTCTCGCCGTTGTAGC-3

gyrB

F:5-TGCAGTGGAACGACAGCTAC-3

142

DNA gyrase, subunit B

R:5-GCGATTTCGTTATCGGTGAT-3

thyA

F:5-CTCGACCTCGTTCGTACCAT-3

149

thymidylate synthase

R:5-GATTTGAACGCGAGCTTCTT-3

rpoB

F:5-CGTTTCGGTGAAATGGAAGT-3

128

DNA-directed RNA polymerase subunit beta

R:5-CCCTTGACCAGGTTTTCGTA-3

pyrG

F:5-AGTCACCCTCCTCAAACTCG-3

170

CTP synthetase

R:5-TCGTGAAGTTGTTGGCCTTG-3

atpD

F:5-TCGAAACGGGTATCAAGGTC-3

142

ATP synthase subunit beta

R:5-TAACCGCCGTGTTCCTTCGC-3

16S rDNA

F:5-GCTCGTGTCGTGAGATGTT-3

156

encoding the 16S rRNA

R:5-TGTGAAGCCCTACCCATAA-3